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Michealis-Menten curve of LmP oxidation of LmCytc. KM and kcat used to determine efficiency (kcat/KM) were calculated using the equation obtained from the Lineweaver-Burk plot (inset). The reaction conditions were: 25 mM potassium phosphate pH 6.5, 50 pM LmP and 0.180 mM hydrogen peroxide.
