. Author manuscript; available in PMC: 2013 Oct 19.

Published in final edited form as: Nat Commun. 2011;2:329. doi: 10.1038/ncomms1331

Table 1.

Discrimination against codon-anticodon mismatches

A-site codon (5' NNN 3')

k_app (s⁻¹) (3 µM ribosomes)

\frac{k_{app (Cognate)}}{k_{app (Near - cognate)}}

Ratio
(htRNA/etRNA)

etRNA

htRNA

etRNA

htRNA

Cognate

UGC

13 ± 1

2.9 ± 0.3

Near-
cognate

1^st position

CGC

4.5 ± 0.4

0.06 ± 0.01

2.9

2^nd position

UAC

0.013 ± 0.003

0.0022 ± 0.0004

1000

1300

1.3

3^rd position

UGG

0.033 ± 0.006

0.010 ± 0.003

390

290

0.7

Bold face letters underlined indicate mismatches.