Table 3.
Cys-tRNACys | kapp (s−1) | [fMet-Cys]/[TC] | Fidelity of proofreading |
||
---|---|---|---|---|---|
UGC codon | CGC codon | UGC codon | CGC codon | ||
etRNA-WT | 1.6 ± 0.2 | 0.0011 ± 0.0001 | 0.46 | 0.40 | 1.2 |
etRNA-eM1 | 0.31 ± 0.06 | Not determined | 0.17 | 0.03 | 5.7 |
Summary of the rate constant kapp of dipeptide formation and the amplitude [fMet-Cys]/[TC] for calculation of fidelity. TC = ternary complex.