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. 2013 Oct 18;8(10):e76955. doi: 10.1371/journal.pone.0076955

Figure 2. Viability of mouse peritoneal cells after challenge with B. cereus ATCC 10876 secreted proteins.

Figure 2

(A) Flow cytometric assessment of the percentage of propidium iodide-stained cells isolated from the peritoneal cavity of mice (n = 4/group) injected with 500 µl of diluted supernatants of B. cereus ATCC 10876 or B. cereus PlcR mutant, or with heat-treated supernatants of B. cereus 10876. Bars labeled with “10876” represent 10 min (dark blue) and 45 min (lighter blue) incubation times after injection. (B) Representative forward vs. side scatter plots of cells retrieved from the peritoneal cavity of mice 45 min after injection with supernatants of B. cereus ATCC 10876 (left panel), the B. cereus PlcR mutant (middle panel), or heat-treated supernatants of B. cereus 10876 (right panel).