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. 2013 Oct 18;8(10):e76955. doi: 10.1371/journal.pone.0076955

Figure 4. B. cereus ATCC 10876 harbors two copies of Hbl-encoding genes.

Figure 4

(A) Schematic operon overview (not to scale) of the two hemolysin BL loci in the genome of B. cereus ATCC 10876. The PATRIC gene annotation numbers (4070–4073 and 1069–1071) are indicated above the genes, and the PlcR-boxes are shown below, with conserved nucleotides of the plcR consensus sequence indicated in bold. Locus hbl-I harbors a second copy of an hbl-A-like gene, hblB, located 375 nucleotides downstream of hblA. An inverted repeat representing a putative transcriptional stop site 96 nucleotides downstream of hblA is indicated. Up- and downstream of the hbl genes in locus hbl-II the genes ORF-14 and ORF-16 having homology to the Bacillus anthracis plasmid pXO1 replication system are shown. (B) Presence of the hbl-II locus in B. cereus ATCC 10876 verified by PCR. The first fragment extended from 438 bp upstream of hblC2 to 85 bp into the coding region of the same gene (total length of the predicted amplicon: 523 bp); the second fragment started at bp 85 in the coding region of hblA2 and extended 318 bp downstream of the operon (total length of the predicted amplicon: 403 bp).