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. 2013 Oct 18;8(10):e76955. doi: 10.1371/journal.pone.0076955

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) CHO cells were incubated for 30 min with different combinations of Hbl and Nhe toxin components (5 nM for Hbl toxins, 3% final concentration of recombinant Nhe toxin-containing sterile culture supernatants) as indicated. Cells treated with 1% Triton-X-100 or PBS (NT) served as positive or negative control, respectively. An additional control received NheA and NheB components only (last column on the right). The 100% viability mark is indicated by the dotted line. B, Hbl-B; L1, Hbl-L₁; L2, Hbl-L₂. (B) Viability assessment of CHO cells incubated with Nhe or Hbl toxins for 30 min in the presence of anti-Hbl toxin component or non-immune (control) serum.