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. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: Methods Enzymol. 2011;492:189–211. doi: 10.1016/B978-0-12-381268-1.00018-5

Table I.

Thermodynamic parameters recovered from the response of tryptophan spectral parameters to denaturant concentration.

Assumed Imax I(330) λmax <λ>310–370 nm <λ>normalized
ΔGw
1
10.0 10.4 10.0 11.9 10.7 10.0
m2 2.5 2.6 2.5 2.8 2.5 2.5
Cm3 4.0 3.9 4.0 4.3 4.3 4.0
1

values are in kcal mol−1

2

values are in kcal mol−1 M−1

3

values are in M