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. Author manuscript; available in PMC: 2014 Sep 18.
Published in final edited form as: Bioconjug Chem. 2013 Aug 16;24(9):1496–1506. doi: 10.1021/bc400018u

Figure 2.

Figure 2

Reductive amination-mediated cross-linking between aldehyde-containing DNA 20-mer (5′-G TCA CTG GTA DHP-deaza-dG CA AGC ATT G-3′) and E. coli AlkB protein visualized via SimplyBlue protein staining. Lane 1: protein size markers; Lane 2: AlkB protein (negative control); Lane 3: DPCs generated using 1:2 ratio of DNA to protein; Lane 4: proteinase K digested reaction mixture from lane 3; Lane 5: DPCs generated using 4:1 molar ratio of DNA to protein; Lane 6: DPCs generated using 1:10 ratio of DNA to protein. Multiple bands observed in the region > 40 kDa are due to protein aggregation.