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Variation of DPC yields with increased protein:DNA molar ratio. 32P-endlabeled DNA strands containing site-specific DHP-deaza-dG were subjected oxidation to aldehyde and reductive amination in the presence of recombinant AlkB protein as described in Figure 1. The molar equivalents of AlkB protein to DNA were varied between 0.5:1 to 5:1, and DPCs were visualized by phosphorimaging. The production of covalent conjugates is increased in the presence excess of excess protein (Lanes 4–5).
