Table 2.
Sites of reductive amination-mediated crosslinking between DHP-deaza-dG containing DNA and recombinant AlkB protein as identified by nano HPLC-ESI+-MS/MS of tryptic digests.
| Amino acid positions | Amino acid sequence | Site of modification | Location within the protein |
|---|---|---|---|
| 25 – 35 | FAFNAAEQLIR | R35 | - |
| 122 – 127 | CVPGAK | K127 | DNA binding groove |
| 122 – 134 | CVPGAKLSLHQDK | K127 | DNA binding groove |
| 161 – 166 | RNDPLK | R161 | DNA binding groove |
| 162 – 166 | NDPLK | K166 | DNA binding groove |
| 162 – 167 | NDPLKR | K166 | DNA binding groove |
| 162 – 183 | RLLLEHGDVVVWGGESR | R167 | DNA binding groove |
| 194 – 204 | VGVHPLTTDCR | R204 | Active site |
| 194 – 210 | VGVHPLTTDCRYNLTFR | R204 | Active site |
| 194 – 210 | VGVHPLTTDCRYNLTFR | R210 | Active site |
| 205 – 215 | YNLTFRQAGKK | R210 | Active site |