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. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: Virology. 2008 Oct 16;382(1):107–114. doi: 10.1016/j.virol.2008.09.008

Figure 4. Effect of 2-OHM on LASV Z-GLuc budding.

Figure 4

293T cells were transfected (quadruplicate) in a 96-well plate in the presence of 2-OHM (100 μM) or vehicle alone (DMSO) at the same concentration present in 2-OHM treated cells. At 48 h post-transfection, cells were measured for viability using CellTiter Glo (Promega) and TCS was collected for GLuc measurement. A. Cell viability. B. Measurement of GLuc activity in TCS. C. Measurement of GLuc activity in cell lysates. D. Comparison of LASV-Z(WT)-GLuc budding and LASV-Z(G2A)-GLuc budding, where budding activity of LASV-Z(WT)-GLuc is set at 100.