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. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: Virology. 2008 Oct 16;382(1):107–114. doi: 10.1016/j.virol.2008.09.008

Figure 5. Z-GLuc fusion is susceptible to knockdown of Tsg101.

Figure 5

Cells were transfected in triplicate wells with siRNA directed against Tsg101, or with a control siRNA. At 24 h post-transfection, cells were transfected to express LASV-Z(WT)-GLuc. TCS was collected for GLuc assay 24 h after LASV-Z(WT)-GLuc transfection. A. Activity of GLuc in TCS when normalized for activity from LASV-Z(WT)-GLuc expressing cells in the presence of non-specific siRNA (set to 1). B. Cellular expression of GLuc when normalized for activity from LASV-Z(WT)-GLuc expressing cells in the presence of non-specific siRNA (set to1). C. Budding efficiency of LASV-Z(WT)-GLuc in the presence of Tsg101-specific siRNA where budding efficiency in the presence of non-specific siRNA was set at 1. Budding efficiency was calculated as follows: GLuc activity (TCS)/([GLuc activity (TCS)]+[ GLuc activity (CL)]).