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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Virology. 2013 Sep 5;446(0):251–259. doi: 10.1016/j.virol.2013.08.013

Figure 1. HPV16 E7 expression interferes with the downregulation of a range of APC/C substrates during mitosis in primary human foreskin fibroblasts (HFFs).

Figure 1

(A) HFFs with stable expression of empty vector (HFF C) or HPV16 E7 (HFF E7) were synchronized with a double thymidine block and then released into fresh medium. Protein extracts were prepared at the indicated time points after release from the second thymidine block and steady state levels of pRB, cyclin A, NEK2A, cyclin B, securin, CDC20, PLK1, Aurora B and actin were assessed by Western blotting. The results of a representative experiment are shown. Similar results were obtained in 3 independent experiments. Mitotic indices as determined by FACS analyses with a parallel set of samples stained with MPM-2 and propidium iodide, are presented below the blots. (B) Quantifications of band intensities of cyclin A, NEK2A, cyclin B and securin normalized to actin for the experiment shown in panel A are plotted. Protein levels in HFF C and HFF E7 populations at the 8 hour time points were each set to 1 even though they were not identical in the two cell populations. (C) Representative immunofluorescence analyses of Ki67 in HFF C and HFF E7. Scale bars represent 10 µm.