Skip to main content
. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Virology. 2013 Sep 5;446(0):251–259. doi: 10.1016/j.virol.2013.08.013

Figure 5. Depletion of EMI1 partially restores degradation kinetics of cyclin A and NEK2A in HPV16 E7-expressing primary human fibroblasts.

Figure 5

(A) HFF C (C) and HFF E7 (E7) were transfected with a pool of EMI1 specific siRNAs (siEMI1) or non-targeting control si RNA (siCtrl) before being subjected to a double thymidine block. Cells were then released into nocodazole and protein extracts were prepared at the time points indicated. Western blot analyses of pRB, CDC27, H3-pS10, EMI1, cyclin A, NEK2A, cyclin B, securin and actin are shown. The asterisk in the CDC27 panel indicates slower migrating, phosphorylated form of CDC27. Similar results were obtained in two additional independent experiments. Mitotic indices, as determined by FACS analyses with a parallel set of samples stained with MPM-2 and propidium iodide, are presented below the blots. (B) Quantifications of band intensities of cyclin A and NEK2A normalized to actin for the experiments shown in panel A are plotted. For each cell population and transfection group protein levels at the 8 hour time points were each set to 1 even though they were not identical in the different populations.