Table 4. Primers used in the validation experiment.

Unigene number	Similarity	RT-PCR and qRT-PCR
		Primers Sequences
unigene18988	chymotrypsin BI	GCCAGCCAGGTCTCCATT
		GGAGTGACGCCGGTCTTCT
unigene8925	ubiquitin-conjugating enzyme E2	CAACGAATCAGCAAGGAG
		CTGGCGGGTCCACTCAC
unigene7508	argonaute 2	TGTAAGGCTCAAACTGGA
		TGGAGCAGCTCTTATCAC
unigene1876	heat shock protein 70	TCTCGGGTCTGAATGTGC
		ACGGGTGATGGAGGTGTA
unigene18451	penaeidin4a	AAGGCGAAGCGTACAGGG
		CGAGCATCTGAGACGGAAA
unigene18880	peroxinectin	ATGCTTTCTATGGACCTCG
		CCACAAACCTTCTAGCCTCT
unigene9038	glucan binding protein	GTTGACTGGACCAAGGAGA
		GAAGAAGCCGTTCGTGCC
unigene9387	peroxiredoxin	AGCCTTCAAGTTCACA
		ATCTAATGCCATATCCT