Backbone 15N-T1, T2, and 1H,15N-NOE measured on a 500 MHz NMR spectrometer. (A) 15N-T1, (B) 15N-T2, and (C) 1H,15N-NOE are reported for apo-Pdx1 (blue) and the Pdx1-DNAcon complex (tan). Experimental uncertainties in the measured parameters are indicated as error bars, which often do not exceed the size of the markers on the plot. The secondary structure from the co-crystal of Pdx1 with a consensus DNA duplex is represented by bars above the figure.