Table 1.

Thermodynamic parameters describing binding of Pdx1-HD to dsDNA derived from natural human promoter elements; measured at 298K in 100 mM cacodylate, pH 7.3, 100 mM KCl. The core TAAT recognition element is shown in bold (except in the E1 element, which lacks it, and is used in this study as a control to describe non-specific binding). All error bars on fitted ITC parameters represent standard error of the mean estimated from triplicate measurements, with fitting performed in Origin 7.0 (MicroCal, Inc.).

DNA	Forward Sequence	n	Kd (nM)	ΔH (kcal mol−1)
Insulin A1	5´-AGGCCCTAATGGGCCA-3´	0.9 ± 0.1	7 ± 2	−10.2 ± 0.1
Insulin A3	5´-AGACTCTAATGACCCG-3´	0.9 ± 0.1	4 ± 1	−9.63 ± 0.05
Insulin E1	5´-AGCCATCTGCCGACCC-3´	1.0 ± 0.1	1300 ± 300	−2.7 ± 0.1
IAPP A1	5´-GGAAATTAATGACAGA-3´	1.1 ± 0.1	22 ± 5	−4.60 ± 0.03
IAPP A2	5´-ATGAGTTAATGTAATA-3´	1.2 ± 0.1	27 ± 7	−5.71 ± 0.05