Table 2.

Thermodynamic parameters describing binding of Pdx1-HD to dsDNA derived from the consensus binding sequence; measured at 298K in 100 mM cacodylate, pH 7.3, 100 mM KCl. The core TAAT recognition element is shown in bold and mutations are highlighted in italics. All error bars on fitted ITC parameters represent standard error of the mean estimated from triplicate measurements, with fitting performed in Origin 7.0 (MicroCal, Inc.).

DNA	Forward Sequence	n	Kd (nM)	ΔH (kcal mol−1)
Consensus	5´-CCACTCTAATGAGTTC-3´	0.9 ± 0.1	5 ± 1	−8.58 ± 0.04
Con. T1→A	5´- CCACTCAAATGAGTTC -3´	1.0 ± 0.1	20 ± 4	−4.98 ± 0.03
Con. A2→T	5´- CCACTCTTATGAGTTC -3´	1.0 ± 0.1	360 ± 50	−3.88 ± 0.04
Con. A3→T	5´- CCACTCTATTGAGTTC -3´	1.0 ± 0.1	110 ± 20	−2.74 ± 0.03
Con. T4→A	5´- CCACTCTAAAGAGTTC -3´	0.9 ± 0.1	64 ± 4	−6.27 ± 0.02
Con. 5´C→G	5´- CCACTGTAATGAGTTC -3´	1.0 ± 0.1	10 ± 2	−7.45 ± 0.03
Con. 5´C→A	5´- CCACTATAATGAGTTC -3´	1.0 ± 0.1	9 ± 1	−7.07 ± 0.02
Con. 5´C→T	5´- CCACTTTAATGAGTTC -3´	0.9 ± 0.1	8 ± 1	−7.00 ± 0.02
Con. 3´G→A	5´- CCACTCTAATAAGTTC -3´	1.1 ± 0.1	11 ± 3	−4.29 ± 0.02
Con. 3´G→T	5´- CCACTCTAATTAGTTC -3´	1.1 ± 0.1	4 ± 1	−8.86 ± 0.03
Con. 3´G→C	5´- CCACTCTAATCAGTTC -3´	1.1 ± 0.1	30 ± 3	−9.08 ± 0.02