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. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: Pharm Res. 2009 Mar 17;26(6):1516–1524. doi: 10.1007/s11095-009-9864-8

Fig. 7.

Fig. 7

Confocal fluorescence images of K562 cell clusters treated with free G4243 or Tf-LP G4243. The cell clusters were treated with 1 µM of free G4243 or Tf-LP G4243 for 6 h and then fixed with 4% paraformaldehyde and stained with DiI and DRAQ5. They were imaged on a laser scan confocal microscope with excitation wavelengths of 488 nm and 543 nm for G4243 and DiI, respectively. The cytoplasm and nuclei were stained with red (DiI) and blue (DRAQ5), respectively. Green fluorescence was derived from FITC-labeled G3139 (G4243), thus indicates the distribution of the ODN within the cell clusters. The images show selected optical sections from the outer layers of cell cluster to the inner layers (from 1–4) a K562 cell cluster treated with free G4243. b K562 cell cluster treated with Tf-LP. Scale bar=20 µm.