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. 2013 Nov;94(5):971–979. doi: 10.1189/jlb.0213080

Figure 5. The influence of Hla on cytokine expression following infection of human blood with USA300.

Figure 5.

(A) Concentrations of IL-1β and IL-8, as determined by ELISA at 5 h postinfection of human blood with 1 × 105 CFU/mL USA300, USA300Δhla (Δhla), USA300Δhla Comp (Comp), DPBS control, or USA300 incubated at ≥98°C for 20 min prior to infection (Heat tx). (B) CBA analysis of IL-6, IL-10, TNF-α, IFN-γ, IL-17A, IL-12p70, IL-2, and IL-4 expression in human blood at 5 h postinfection with 1 × 105 CFU/mL USA300, USA300Δhla, USA300Δhla Comp, or DPBS control. Data represent at least four separate experiments using at least three different blood donors, with *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001, as determined by one-way repeated-measures ANOVA with Tukey's post-test relative to samples infected with USA300.