Figure 4. CD27⁻ and CD27⁺ developing B cell compartments showed equally diversified V_H gene usage.

V_HDJ_H rearrangements were amplified by nested PCR with cDNA obtained from CD19⁺CD27⁻ and CD19⁺CD27⁺ developing B cell compartments in 19 wk FL. After PCR, the amplicands were cloned into bacteria and sequenced, and the V_H gene sequences were compared to reference sequences to obtain V_H gene segment identity. The percentage of V_H gene segments within each V_H gene family from CD19⁺CD27⁻ pro-B (n=30), CD19⁺CD27⁻ pre-B (n=17) and CD19⁺CD27⁺ pro/pre-B cells (n=28) are shown.