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. 2013 Nov;94(5):953–962. doi: 10.1189/jlb.0313174

Figure 5. Effect of Rac2 on Sp1 phosphorylation.

Figure 5.

(A) Effect of Rac2 overexpression on Sp1 activation/phosphorylation. RAW264.7/LR5 cells were transfected with increasing Rac2 DNA, and resulting Western blots were performed that were stained with the indicated antibodies. (B) Schematic for potential Sp1 binding to PLD2 promoter. Shaded in yellow are putative Sp1-binding sites in the PLD2 promoter. (C) Effect of silencing Sp1 on PLD2 expression. (D) Effect of prolonged MCP-1 stimulation on Sp1-mediated rescue of PLD2 protein expression. (E) Overexpression of β-catenin reverses the negative effect of Rac2 on PLD2 expression. (F) β-Catenin is activated at early times of incubation with MCP-1/CCL2 but not at later (>1 h) times. RAW264.7/LR5 cells were stimulated with 5 nM MCP-1.