Table 1.
TMP-tags with various fluorophores for live cell imaging.
| Chemical Tag | Fluorophore | Protein of Interest | Reference |
|---|---|---|---|
|
Non-covalent
TMP-tag |
BODIPY texas red | Plasma membrane (inner surface); cell nucleus. |
(Miller et al. 2005) |
| Fluorescein (protected) |
Myosin light chain (MLC); cell nucleus. |
(Miller et al. 2005, Cai et al. 2010) | |
| Hexachlorofluorescein (protected) |
Myosin light chain kinase; α-tubulin. |
(Calloway et al. 2007) | |
| Atto655 | Histone protein 2B (H2B). |
(Wombacher et al. 2010) | |
| Cy3 | Spliceosome in yeast cell extract. |
(Hoskins et al. 2011) | |
| Terbium complex | Plasma membrane (outer surface). |
(Rajapakse et al. 2009) | |
|
| |||
|
Covalent
TMP-tag |
Fluorescein (protected) |
H2B; TOMM20; MLC, α- actinine. |
(Gallagher et al. 2009, Chen et al. 2012) |
| Dapoxyl | H2B; plasma membrane. |
(Chen et al. 2012) | |
| Atto655 | H2B; plasma membrane. |
(Shaner et al. 2007) | |
|
| |||
|
Fluorogenic
(and covalent) TMP-tag |
Fluorescein (protected) |
H2B; plasma membrane (inner surface). |
(manuscript in revision) |
| Atto520 | H2B; TOMM20; MLC, α- actinine. |
(manuscript in revision) | |