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. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: Magnes Res. 2010 Oct 25;23(4):S199–S206. doi: 10.1684/mrh.2010.0218

Figure 3.

Figure 3

Effect of NMDA receptor blockade (MK-801, 0.5 mg/kg/day, s.c.) on PMN (superoxide production) activity during 3 wks of severe MgD treatment. Whole blood PMNs were obtained using a modified ficoll hypaque reagent and a step-gradient centrifugation.18 Basal and stimulated superoxide anion production was assayed in the absence or presence of 100 ng/ml phorbol myristate acetate plus 75 µM cytochrome c with or without 50 µg SOD. Superoxide production was estimated as SOD-inhibitable reduction of cytochrome c using the extinction coefficient: E550 = 2.1×104M−1cm−1. Values are means ± SD from 3–5 rats/group.