Skip to main content
. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: Magnes Res. 2010 Oct 25;23(4):S199–S206. doi: 10.1684/mrh.2010.0218

Figure 5.

Figure 5

Effect of the NEP inhibitor, phosphoramidon (PR: 5 mg/kg/day, s.c.) on plasma SP levels during week 1 of severe MgD. SP levels were determined using an ELISA kit from R&D Systems (Minneapolis, MN).31 The assay is a competitive binding technique in which sample SP competes with a fixed amount of horseradish-labeled SP for sites on a murine monoclonal antibody. Color development is inversely proportional to SP concentration and absorbance is read at 450 nm. Values were normalized to baseline and are means ± SE of 3–6 rats. To obtain total levels for week 1, time-courses were area integrated.