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. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: Magnes Res. 2010 Oct 25;23(4):S199–S206. doi: 10.1684/mrh.2010.0218

Figure 6.

Figure 6

Effect of extending exposure to 5 wks on plasma endotoxin (LPS) levels during severe MgD with or without neurokinin-1 (NK-1) receptor blockade (L-703,606; 0.5 mg/kg/day, s.c. pellet). Systemic endotoxemia was measured in blood samples obtained with endotoxin-free needles, syringes, and glassware, then diluted 1:10 in endotoxin-free water, and heated for 5 min to remove interfering plasma components. Endotoxin was determined using limulus amebocyte lysate.34 A quantitative colorimetric “Pyrochrome” assay (Associates of Cape Cod, Woods Hole, MA) was performed in 96-well microplates and read at 540 nm using a plate reader (Versa max; Molecular Devices). The detection limit for “Pyrochrome” is 0.3 pg/ml. Values are means ± SE of 3–5 rats.