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. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: J Biol Chem. 2002 Aug 20;277(43):40583–40593. doi: 10.1074/jbc.M203292200

Fig. 2. Sequence requirements for the in vitro transport of CYP2E1 protein.

Fig. 2

35S-Labeled translation products generated in the presence or absence of added PKA were used for the import into rat liver mitochondria. Mitochondria from a companion set of reactions were treated with trypsin (300 µg/ml), and the proteins were resolved by SDS-PAGE on a 12% gel and subjected to fluorography. A–C, wild-type and various mutant CYP2E1 proteins were used as indicated. In D, mitochondria were pre-incubated for 20 min on ice with or without added 2,4-dinitrophenol (2,4DNP, 25 µm), carbonyl cyanide-m-chlorophenyl hydrazone (CCCP, 25 µm), or in the absence of added energy mix (E mix) before initiating import reaction with phosphorylated wild-type CYP2E1 translation product. The percentage binding and import are presented.