Table 1. The sequences of the primers utilized in study.

Target	Sequences
IL-18	Forward 5'-GAAACGTCAATAGCCAGTTGC-3'
	Reverse 5'-TCCCATGCTCTTTCTCACAACA-3'
IL-10	Forward 5'-AGCAGATCAAGGAGACGTTC-3'
	Reverse 5'-ATCAGCAGGTACTCCTCGAT-3'
IL-8	Forward 5’-TATCACTGGAGAGTCTCGCTGTC-3’
	Reverse 5’-TCAGTCCTCCTGCAGTGTATACC-3’
IFN-γ	Forward 5' - ACACTGACAAGTAAAGCCGCACA-3'
	Reverse 5' -AGTCGTTCATCGGGAGCTTGGC-3'
β-actin	Forward 5'-CAACACAGTGCTGTCTGGTGGTA-3'
	Reverse 5'-ATCGTACTCCTGCTTGCTGATCC-3'

Specific primers for chicken IFN-γ, IL-18, IL-8 were described by Abdul-Careem et al [1], and for IL-10, and β-actin were described by Abdul-Careem et al [2].