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. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: J Immunol. 2011 Jan 31;186(5):3226–3236. doi: 10.4049/jimmunol.1003587

Figure 6. Ex-vivo production of MMP-9 and IL-6 following incubation of the fetal membranes with sgp130, sIL-6R and LPS.

Figure 6

Recombinant sIL-6R and LPS stimulated the release of MMP-9 (A). Addition of recombinant sgp130 over sIL-6R or LPS reversed the release of MMP-9. Incubation of the tissues with sgp130, sIL-6R and their combination had no effect of IL-6 release in the conditional media of the amniochorion explant (B). The IL-6 levels were significantly upregulated following incubation with LPS. sgp130 did not impact the stimulatory effect of LPS. These results indicate that sIL-6R and sgp130 modulate MMP-9 activity through mechanisms independent of IL-6 levels in the system. Data presented as mean + SEM of 7 independent experiments and analyzed by one-way ANOVA followed by post-hoc Student-Newman-Keuls tests. Means with at least one common superscript are not statistically significant (P > 0.05).