Table 1.
Only VGLUT3 mRNA exhibits changes after axotomy of the sciatic nerve. Percentage of DRG neurons expressing either VGLUT1, VGLUT2 or VGLUT3 transcript, after 7-day sciatic nerve axotomy or 1- or 3-day CFA injuries. Sham animals were also included. To provide the global percentage of neurons expressing each VGLUT in normal conditions, the values from contralateral DRGs in sham animals were combined and averaged accordingly.
| VGLUT1 |
VGLUT2 |
VGLUT3 |
||||
|---|---|---|---|---|---|---|
| Ipsi | Contra | Ipsi | Contra | Ipsi | Contra | |
| 7-day Axotomy | 42.1 ± 0.4 (n=4) | 42.5 ± 0.9 (n=4) | 66.5 ± 1.7 (n=4) | 69.3 ± 1.0 (n=4) | 12.8 ± 0.4 (n=5)* | 17.8 ± 1.6 (n=5) |
| Sham | 40.3 ± 1.0 (n=4) | 42.8 ± 1.4 (n=4) | 68.9 ± 1.0 (n=4) | 65.7 ± 1.8 (n=4) | 15.1 ± 0.5 (n=5) | 15.7 ± 1.1 (n=5) |
| 1-day Inflammation | 52.2 ± 0.6 (n=3) | 51.5 ± 1.6 (n=3) | 78.7 ± 1.6 (n=3) | 76.1 ± 0.8 (n=3) | 16.1 ± 0.9 (n=5) | 14.4 ± 0.3 (n=5) |
| Sham | 51.3 ± 2.4 (n=3) | 52.8 ± 0.8 (n=3) | 78.9 ± 1.7 (n=3) | 76.6 ± 2.0 (n=3) | 14.0 ± 0.7 (n=5) | 17.6 ± 1.4 (n=5) |
| 3-day Inflammation | 38.4 ± 0.8 (n=4) | 41.8 ± 1.8 (n=4) | 64.9 ± 1.8 (n=4) | 66.0 ± 2.5 (n=4) | 16.4 ± 0.9 (n=5) | 17.6 ± 1.3 (n=5) |
| Sham | 41.0 ± 1.2 (n=3) | 38.8 ± 0.9 (n=3) | 62.3 ± 3.2 (n=3) | 64.7 ± 1.8 (n=3) | 16.1 ± 1.2 (n=5) | 18.4 ± 0.6 (n=5) |
|
| ||||||
| Global Percentage | 44.7 ± 4.2 (n=10) | 69.1 ± 3.7 (n=10) | 17.1 ± 0.7 (n=15) | |||
Values are expressed as mean ± SEM.
*
(P<0.05) when comparing contra- vs. ipsilateral DRGs after a 7-day axotomy.