Table 3.
Cell surface antigens that are commonly used for the flow cytometric analyses of mouse immune cells.
| Antigen Name | Other Name | Cells |
|---|---|---|
| CD3 | T, NKT | |
| CD4 | T, DC, Mono | |
| CD8 | T, DC | |
| CD11b | Mac-1, Itgam | Mac, Mono, Neu, NK, T, B |
| CD11c | Itgax | DC, Mono, Mac, NK, T, B, Grn |
| CD14 | LPS receptor | Mac, Mono, Grn, DC |
| CD16 | FcγRIII | Mac, Neu, Mast, NK, NKT, B |
| CD19 | B4 | B, Follicular DCs |
| CD64 | FcγRI | Mono, Mac, DC, Grn |
| CD68 | Mac, Mono, DC (low) | |
| CD83 | DC, T, B | |
| CD115 | CSF-1 receptor | Mono, Mac |
| CD205 | DEC-205 | DC |
| CD206 | Mrc-1, Macrophage man nose receptor C Type 1 | Mac, Mono, DC |
| CD209 | DC-Sign | DC |
| CD301 | MGL-1/2 | Mac, DC |
| B220 | CD45R | B, DC, Mono, T, B, NK |
| F4/80 | EMR-1 | Mac, Mono (low), Neu (low) |
| MHC II | DC, B, Mac, mono, T | |
| MK1.1 | CD161 | NK, NKT |
| Gr-1 | Ly6C/G | Neu, Mono, Mac, T, B, DC |
| Ly6B | 7/4 | Neu, Mono |
| Ly6C | Mono, Mac, Neu, T, B, DC | |
| Ly6G | Neu |
It should be noted that this table is only for the general guidance. While some of markers are highly specific for the cell types (e.g., CD3 and CD19), some of markers are less specific (e.g., CD11c for ATMs). Hence, each investigator should carefully analyze the expression of markers for the target cells for individual experiment. Markers with bold fonts represent the cell types that are commonly defined by these markers. T, T cells; B, B cells; NKT, NKT cells; DC, Dendritic cells (DCs); Mac, macrophages; Mono, monocytes; Neu, Neutrophils; NK, NK cells; Grn, granulocytes; low, low expression levels that can be differentiated from the cells expressing high levels by flow cytometric analyses.