Table 2.

Comparison of the inhibition on interferon-γ (IFN-γ) -stimulated gene expression by anti-MD-2 and anti-IFNγR antibodies

	Ct				Fold change compared with control
Gene name	Control	IFN-γ	IFN-γ in the presence of anti-IFN-γR	IFN-γ in the presence of anti-MD-2	By IFN-γ	By IFN-γ in the presence of anti-IFN-γR	By IFN-γ in the presence of anti-MD-2
MMP-1	27·33	25·88	26·58	27·62	2·73	1·68	0·82
MMP-8	26·98	25·90	26·29	27·05	2·11	1·61	0·95
MCP-1	24·95	22·25	24·23	25·88	6·50	1·65	0·52
CXCL10	27·84	25·31	27·00	27·23	5·78	1·79	1·53
ICAM-1	25·99	24·70	25·89	25·94	2·43	1·07	1·04
VCAM-1	27·96	26·97	27·36	28·60	1·99	1·52	0·64
Interferon regulatory factor-1	28·76	26·59	27·70	28·22	4·5	2·08	1·45
MD-2	27·14	26·04	26·84	27·58	2·14	1·23	0·74
TLR10	27·73	26·62	27·04	28·56	2·17	1·61	0·56

U937 cells were treated with 100 units/ml of IFN-γ in the absence or presence of 10 μg/ml of anti-IFN-γR or anti-MD-2 antibodies for 24 hr. After the treatment, RNA was isolated and PCR array was performed as described in Materials and methods. The threshold cycle (Ct) is the cycle at which the amplification plot crosses the threshold. Smaller Ct means higher gene expression. The Ct of the all gene expression was normalized to that of GAPDH.