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. 2013 Oct 3;140(3):314–322. doi: 10.1111/imm.12139

Figure 1.

Figure 1

Down-regulation of miR-301a expression in primary macrophages and RAW264.7 cells stimulated with Toll-like receptor (TLR) agonists. Mouse peritoneal macrophages (a–c) and RAW264.7 cells (d–f) were stimulated with different concentrations of lipopolysaccharide (LPS) for 24 hr (a and d) or at the indicated time-points with 100 ng/ml LPS (b and e), and 10 μg/ml CpG or poly (I:C) for 24 hr (c and f). The expression of miR-301a was measured using quantitative PCR and normalised to the expression of U6. Data are presented as the mean ± SD (n = 3) of three independent experiments. *P < 0·05; **P < 0·01.