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. 2013 Oct 3;140(3):323–334. doi: 10.1111/imm.12140

Figure 3.

Figure 3

Interleukin-17A (IL-17A) enhances the stability of bacillus Calmette–Guérin (BCG) -induced inducible nitric oxide synthase (iNOS) mRNA. Macrophages were pre-treated with IL-17A (25 ng/ml) for 24 hr, followed by BCG infection at a multiplicity of infection (MOI) = 1 for 3 hr. Then, the infected macrophages were washed with PBS and replenished with fresh medium containing 1 μg/ml actinomycin D. At indicated time-points, total RNA from macrophages were extracted for reverse transcription and subjected to quantitative PCR analysis of iNOS mRNA expression. The iNOS mRNA expression level at 0 min post-actinomycin D treatment was set as 100%. The half-life of iNOS mRNA was defined as the time required for iNOS mRNA to degrade until 50% remained. The data represented mean ± SE of seven independent experiments.