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. 2013 Oct 3;140(3):323–334. doi: 10.1111/imm.12140

Figure 5.

Figure 5

Interleukin-17A (IL-17A) enhances the clearance of intracellular bacillus Calmette–Guérin (BCG). (a) Phagocytosis of BCG by macrophages with or without IL-17A pre-treatment. The macrophages were pre-treated with IL-17A (25 ng/ml) for 24 hr, followed by BCG infection at a multiplicity of infection (MOI) = 1. After 2 hr of BCG infection, non-phagocytosed bacteria were washed. Intracellular BCG was recovered by lysis of the macrophages and plated onto 7H10 agar. Colony-forming units (CFU) were enumerated after 3 weeks of incubation. The data represented mean ± SE of three independent experiments. N.S., not significant. (b) Intracellular survival of BCG in macrophages with or without IL-17A pre-treatment. The macrophages were pre-treated same as (a). After 48 hr of BCG infection, non-phagocytosed bacteria were washed. Intracellular BCG was recovered by lysis of the macrophages and plated onto 7H10 agar. CFU were enumerated after 3 weeks of incubation. The data represented mean ± SE of five independent experiments. *P < 0·05.