Skip to main content
. 2013 Oct 3;140(3):352–361. doi: 10.1111/imm.12147

Figure 3.

Figure 3

Effect of Pam3CSK4 on interferon-γ (IFN-γ) -induced interferon regulatory factor 1 (IRF1) expression. (a) END-D cells were pre-treated with or without Pam3CSK4 at 10 μg/ml for 1 hr and then stimulated with IFN-γ at 100 ng/ml for 2 hr. The expression of IRF1 protein was determined by immunoblotting. (b) Cells transfected with inducible nitric oxide synthase (iNOS) -luciferase reporter gene were pre-treated with or without Pam3CSK4 at 10 μg/ml for 1 hr and then stimulated with IFN-γ at 100 ng/ml for 6 hr. The iNOS-dependent luciferase activity in the cell lysates was determined with a luminometer. *P < 0·01 versus IFN-γ alone.