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. 2013 Oct 3;140(3):352–361. doi: 10.1111/imm.12147

Figure 4.

Figure 4

Effect of Pam3CSK4 on interferon-γ (IFN-γ) -induced phosphorylation of signal transducer and activator of transcription 1 (STAT1) at Y701. (a) END-D cells were pre-treated with or without Pam3CSK4 at 10 μg/ml for 1 hr and then stimulated with IFN-γ at 100 ng/ml for 2 hr. (b) Cells were pre-treated with or without Pam3CSK4 at 10 μg/ml for 1 hr and then stimulated with IFN-γ at 100 ng/ml for various hours. The expression of STAT1 and phosphorylated Y701 (p-Y701) STAT1 was determined by immunoblotting. A typical experiment of three independent experiments is shown.