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Effect of Pam3CSK4 on interferon-γ (IFN-γ) -induced Janus kinase 1/2 (JAK1/2) activation. END-D cells were pre-treated with or without Pam3CSK4 at 10 μg/ml for 1 hr and then stimulated with IFN-γ at 100 ng/ml for 0·5 hr. The expression of phosphorylated JAK1 (p-JAK1) and JAK2 (p-JAK2) was determined by immunoblotting. A typical experiment of three independent experiments is shown.
