Figure 8.

Effect of Pam3CSK4 and interferon-γ (IFN-γ) on the physical association between MyD88 and interferon-γ receptor α (IFN-γRα). (a) END-D cells were stimulated with Pam3CSK4 at 10 μg/ml and/or IFN-γ at 100 ng/ml for 4 hr. (b) RAW 264.7 cells were stimulated with Pam3CSK4 (10 μg/ml), poly I:C (10 μg/ml) or lipopolysaccharide (LPS; 10 μg/ml) for 2 hr. Cell lysates were immunoprecipitated (IP) with anti-IFN-γRα antibody and the immunoprecipitates were immunoblotted (IB) with anti-MyD88 antibody. Cell lysates were also analysed with anti-IFN-γRα, anti-MyD88 and anti-actin antibodies.