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. 2013 Oct 3;140(3):352–361. doi: 10.1111/imm.12147

Figure 9.

Figure 9

Effect of MyD88 small interfering RNA (siRNA) on interferon-γ (IFN-γ) -induced nitric oxide (NO) production in pre-treatment with or without Pam3CSK4. END-D cells were transfected with MyD88 siRNA or control siRNA for 24 hr. The expression of MyD88 protein was determined by immunoblotting (top). MyD88 siRNA and control siRNA-transfected cells were pre-treated with Pam3CSK4 at 10 μg/ml for 1 hr and then stimulated with IFN-γ at 100 ng/ml for 24 hr. The nitrite concentration was determined for NO production (bottom). *P < 0·01 versus control siRNA.