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The epitope targeted by Ab63, N213 and N62 is contained within one O-antigen (OAg) tetrasaccharide repeat. Purified Francisella tularensis lipopolysaccharide (LPS) oligosaccharides were used to compete with the binding of Ab63, N213, N62, FB11 or Ab52 to F. tularensis LPS coated on ELISA plates. Bars denote standard deviation of the mean from two or three experiments. –, 50% inhibition was not reached at the highest available competitor concentrations.
