Figure 2. Effect of Neto1 on GluK2/GluK5 heteromeric receptors.

A, cells were transiently transfected with GluK2/GluK5 alone or plus Neto1. Whole-cell currents are shown in response to glutamate applied for 5 s (continuous line) at the concentration indicated to cells voltage-clamped at −70 mV. B, the peak current response was measured and normalized to the maximum response for each cell. Symbols indicate the mean ± SEM (n= 5 cells) of the response of GluK2/GluK5 receptors with (open squares) or without (filled squares) Neto1. Averaged data were fit with a four-parameter logistic equation (solid or dashed line). C, the extent of desensitization was measured by subtracting the steady-state /peak current ratio from 1 and multiplying by 100 to give percentage desensitization. Steady-state current was measured at the end of the 5 s application. Symbols show mean ± SEM (n= 5 cells). D, the amplitude of the steady-state current was measured at the end of the 5 s application and normalized to the maximum steady-state current for each cell. Symbols show mean ± SEM (n= 5 cells). E, 10 μm or 10 mm glutamate was rapidly applied for 1 s (continuous line) to outside-out patches from cells transfected with either GluK2/GluK5 alone or GluK2/GluK5 + Neto1. Patches were voltage-clamped at −70 mV.