Figure 3. Effect of Neto1 on recovery from desensitization.

A, outside-out patches were excised from cells transfected with the subunits indicated. Pairs of 100 ms applications of 10 mm glutamate (continuous lines) were applied 1 s apart to patches voltage-clamped at −70 mV. B, paired pulses of 10 mm glutamate (100 ms) were applied to outside-out patches at intervals ranging from 100 ms to 5 s. The peak amplitude of the 2nd response was divided by that of the 1st response and multiplied by 100 to give percentage recovery. Data were fitted with a single exponential shown by the continuous line (GluK2) or dashed line (GluK2/GluK5).