A, uEPSC failure rate during the trains at 10 Hz in control and 10 μM CCh. Data are mean ± SEM (n= 7). B, uEPSC failure rate at the steady-state from experiments as shown in A for 7.5, 10 and 20 Hz trains in control and 10 μM CCh. The steady-state failure rate values at 2.5 Hz are the failure rates of the first uEPSC of the trains. For 7.5, 10 and 20 Hz the steady-state failure rate was computed by averaging the failures for the last three uEPSCs in the trains. Results are mean ± SEM, #P < 0.05 control vs. CCh at the same frequency. For control values, individual groups not sharing the same upper case letter are significantly different, P < 0.05 post hoc comparisons with Tukey test. RM-ANOVA (control vs. CCh: F1,38= 7.754, P= 0.008; control vs. frequency: F3,38= 3.183, P= 0.035; CCh vs. frequency: F3,38= 0.658, P= 0.583).C,relative failure rate during the trains (failure rate of the first uEPSC of the train over the uEPSC failure rate in the steady-state) in the absence (control) and presence of 10 μM CCh (CCh) for7.5, 10 and 20 Hz trains. Data are shown as mean ± SEM, *P < 0.05 (paired t test).