Transfection with the Kv2.1 DN did not lead to changes in passive properties or properties of single APs. A, superimposed representative APs from a non-transfected (Ctl) layer 2/3 pyramidal cell and Kv2.1 DN cell from the same slice and layer. We found no differences between Kv2.1 DN and non-transfected control groups for any measured parameters of single APs (amplitude, threshold, dV/dt, half-width: APs elicited with suprathreshold 10 ms current injection). B, traces from a different pair of non-transfected cell (left) and Kv2.1 DN cell (right) indicate no changes in input resistance in the Kv2.1 DN cells. C, left: bar chart indicating no significant differences for input resistance between Kv2.1 DN and non-transfected (Ctl) cells. Right: rheobase did not differ in the Kv2.1 DN group vs. non-transfected (Ctl: n= 12 pairs of cells).