Table 2.
Passive properties and single action potentials (10–20 ms current injection)
| RMP (mV) | Rin (MΩ) | AP (mV) | Vth (mV) | AP HW (ms) | dV/dt up (V s−1) | dV/dt down (V s−1) | |
|---|---|---|---|---|---|---|---|
| NT | −70 ± 2 (23) | 145 ± 10 (23) | 90 ± 2 (23) | −44 ± 0.8 (23) | 1.74 ± 0.1 (23) | 211 ± 14 (23) | 54 ± 6 (23) |
| Kv2.1 DN | −71 ± 2 (23) | 166 ± 17 (23) | 91 ± 2 (23) | −43 ± 1.0 (23) | 1.76 ± 0.1 (23) | 213 ± 15 (23) | 53 ± 5 (23) |
| GFP NT | −67 ± 2.2 (17) | 130 ± 14 (17) | 92 ± 2 (17) | −43 ± 0.8 (17) | 1.56 ± 0.2 (17) | 251 ± 18 (17) | 54 ± 4 (17) |
| GFP alone | −70 ± 2.2 (17) | 154 ± 22 (17) | 92 ± 2 (17) | −43 ± 0.8 (17) | 1.54 ± 0.1 (17) | 243 ± 21 (17) | 55 ± 4 (17) |
| Kv2.1 WT NT | −69 ± 1.8 (6) | 162 ± 27 (6) | 95 ± 1.6 (6) | −45 ± 1.4 (6) | 1.73 ± 0.15 (6) | 228 ± 14 (6) | 50 ± 10 (6) |
| Kv2.1 WT | −67 ± 1.3 (6) | 199 ± 42 (6) | 88 ± 6 (6) | −42 ± 1.2 (6) | 1.53 ± 0.1 (6) | 175 ± 31 (6) | 54 ± 6 (6) |
There were no significant statistical differences between groups. Data are presented as mean ± SEM (number of cells). Non-transfected cells were paired with experimental treatments by selecting a non-transfected cell in the same layer in the same slice (within ∼50 μm). Kv2.1 DN = Kv2.1 pore mutant dominant negative construct. GFP = transfected with DNA for GFP only. GFP NT = paired non-transfected cells for GFP alone group. Kv2.1 WT = wild-type Kv2.1 (overexpression). Kv2.1 WT NT = non-transfected cells paired with WT overexpression group. RMP = resting membrane potential. Rin= input resistance. AP = action potential. Vth= voltage threshold for AP. HW = half width of AP (see Methods). dV/dt= maximum of first derivative of voltage for AP upstroke (up) or repolarization (down). 1 dV/dt of AP are reported in V/s.