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. Author manuscript; available in PMC: 2014 Oct 14.
Published in final edited form as: Dev Cell. 2013 Sep 26;27(1):47–59. doi: 10.1016/j.devcel.2013.08.002

Figure 2. Dppa2 depletion compromises nuclear envelope integrity and leads to disorganized DNA replication.

Figure 2

(A) Visualization of lamin B3 and nuclear pore complexes (mAb414) by immunofluorescence.

(B) Quantification of integrated fluorescence intensity from (A). Bars represent mean and standard error from > 30 nuclei per sample and are representative of 3 independent experiments.

(C) DNA replication assayed by total incorporation of [α-33P]dCTP at the indicated time points. Data shown are mean and standard error from 3 independent experiments.

(D) DNA replication visualized by incorporation of Cy3-dUTP.

(E) Quantification of integrated fluorescence intensity from (D). Bars represent mean and standard error from > 30 nuclei per sample and are representative of 3 independent experiments.

(F) Histone H1 kinase assay was performed at 0 and 20 min after calcium addition.

Scale bars, 10 μm.

See also Figure S2.