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. Author manuscript; available in PMC: 2014 Oct 14.
Published in final edited form as: Dev Cell. 2013 Sep 26;27(1):47–59. doi: 10.1016/j.devcel.2013.08.002

Figure 4. CPC depletion rescues ΔDppa2 phenotypes.

Figure 4

(A) CPC was depleted from extracts using anti-INCENP antibodies (Sampath et al., 2004) and depletion efficiency assessed by Western blotting. * indicates non-specific reactivity.

(B) Sperm nuclei were added together with calcium to metaphase extracts containing rhodamine-labeled tubulin (red). Sperm-associated asters were fixed after 15 min and stained with Hoechst 33342 (blue).

(C) Quantification of tubulin intensity from (B). Bars indicate mean and standard error of > 30 asters from each sample and are representative of 3 independent experiments.

(D) Sperm nuclei were added together with calcium to metaphase extracts supplemented with GST-GFP-NLS. Nuclei were fixed and visualized with Hoechst 33342.

(E) Quantification of nuclear area and roundness from (D). Bars indicate mean and standard error of > 30 nuclei from each sample and are representative of 3 independent experiments.

Scale bars, 10 μm.

See also Figure S4.