Figure 4.

Time courses of recombinant Bla g 7 (rBla g 7) induced I-κB and NF-κB activation in dendritic cells (DCs). (a, c) rBla g 7 at 1 μg/mL was incubated with DCs at 37°C for 0, 20, 40, and 60 min, respectively. Protein levels were detected by immunoblot analysis with specific antibodies. Western blots at the top of each panel are from a typical experiment. (b, d) Bar graphs are the quantified results expressed as mean ± SEM of I-κB, p-I-κB, NF-κB, and p-NF-κB levels from three independent experiments. *P < 0.05 versus 0 min.