Figure 6.

Inhibition of rBla g 7-stimulated dendritic cell (DC)s induced Th2 polarization by antibodies against TIM4, CD80, and CD86. In (a), isolated CD4+ T cells were cocultured with DCs treated with rBla g 7 (1 μg/mL) alone (rBla g 7-DCs) or treated with rBla g 7 and anti-TIM4 (10 ng/mL, rBla g 7-anti-TIM4), anti-CD80 (rBla g 7-anti-CD80) or anti-CD86 antibodies (rBla g 7-anti-CD86) before flow cytometry analysis of intracellular expression of IFN-γ and IL-4 being performed. (b) The ratio of IL-4+ CD4+ T cells versus IFN-γ+ CD4+ T cells. (c) Levels of IL-13 in the supernatants of DCs determined by ELISA. The data were represented as the mean ± SEM for four separate experiments. *P < 0.05 in comparison with medium alone control. ^† P < 0.05 compared with the response to the corresponding uninhibited control.